expression vector pact2 Search Results


pact2  (Becton Dickinson)
90
Becton Dickinson pact2
Pact2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/pact2/pmc02312457-37-45-50
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pact2 - by Bioz Stars, 2026-10
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bacterial expression vector pgex 4t 1  (Danaher Inc)
96
Danaher Inc bacterial expression vector pgex 4t 1
Bacterial Expression Vector Pgex 4t 1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/PGEX-4T-1/10__1074_slash_jbc__m500391200-73-27-31
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gst pdip38 fusion protein expression vector  (Danaher Inc)
96
Danaher Inc gst pdip38 fusion protein expression vector
Gst Pdip38 Fusion Protein Expression Vector, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/Fusion/10__1074_slash_jbc__m208694200-68-3-29
Average 96 stars, based on 1 article reviews
gst pdip38 fusion protein expression vector - by Bioz Stars, 2026-10
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expression library of random primed c-dna from e.histolytica  (Vertis Biotechnologie)
90
Vertis Biotechnologie expression library of random primed c-dna from e.histolytica
Expression Library Of Random Primed C Dna From E.Histolytica, supplied by Vertis Biotechnologie, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/expression+library+of+random+primed+c+dna+from+e+histolytica/pmc02824750-224-8-12
Average 90 stars, based on 1 article reviews
expression library of random primed c-dna from e.histolytica - by Bioz Stars, 2026-10
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pegfp-n1 expression vector  (Becton Dickinson)
90
Becton Dickinson pegfp-n1 expression vector
Pegfp N1 Expression Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/pegfp+c1/pm18511602-67-27-30
Average 90 stars, based on 1 article reviews
pegfp-n1 expression vector - by Bioz Stars, 2026-10
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klenow fragment  (New England Biolabs)
99
New England Biolabs klenow fragment
Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/Klenow+Fragment/custom%40m0212%4033749700
Average 99 stars, based on 1 article reviews
klenow fragment - by Bioz Stars, 2026-10
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pact2.2gtwy  (Addgene inc)
92
Addgene inc pact2.2gtwy
Pact2.2gtwy, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/pACT2%2E2gtwy+(Plasmid+%2311346)/pmc08086697-49-15-35
Average 92 stars, based on 1 article reviews
pact2.2gtwy - by Bioz Stars, 2026-10
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bacterial expression vector pgex 4t 2  (GE Healthcare)
95
GE Healthcare bacterial expression vector pgex 4t 2
Bacterial Expression Vector Pgex 4t 2, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/PGEX-4T-2/10__1074_slash_jbc__m513280200-81-37-41
Average 95 stars, based on 1 article reviews
bacterial expression vector pgex 4t 2 - by Bioz Stars, 2026-10
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pgex5x 3 vector  (GE Healthcare)
95
GE Healthcare pgex5x 3 vector
Pgex5x 3 Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/PGEX-5X-3/10__1074_slash_jbc__m208694200-68-27-29
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pgex5x 3 vector - by Bioz Stars, 2026-10
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bd-hlair-1 fusion protein  (Becton Dickinson)
90
Becton Dickinson bd-hlair-1 fusion protein
Bd Hlair 1 Fusion Protein, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/bd+hlair+1+fusion+protein/pm16380958-161-7-7
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pgl3-basic vector  (Promega)
90
Promega pgl3-basic vector
Pgl3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/pgl3+basic/pm10362257-183-46-51
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pgbkt7 vector containing rilp fused to the gal4 dna-binding domain  (Becton Dickinson)
90
Becton Dickinson pgbkt7 vector containing rilp fused to the gal4 dna-binding domain
<t>RILP</t> interacts with RalGDS. ( a <t>)</t> <t>AH109</t> yeast cells expressing pGBKT7-RILP, pGBKT7-RILP(1–198) or pGBKT7-RILP(199–401) was mated with Y187 yeast cells expressing pACT2-RalGDS(237–914), respectively. Growth on DDO (-Leu/-Trp) media indicated diploids expressing both plasmids. Growth on QDO (-Leu/-Trp/-His/-Ade) media indicated positive interaction between two hybrid proteins. ( b ) The endogenous RalGDS in MCF cell lysates binds to GST-RILP fusion protein as revealed by GST-pulldown using immobilized GST-RILP. ( c ) MCF7 cells were transfected with myc-RalGDS, and the resulted cell lysates were subjected for GST-pulldown assays using GST-RILP, GST-RILP(1–198) or GST-RILP(199–401), respectively. The results showed that RalGDS binds to full-length RILP and N-terminal (1–198) but not C-terminal (199–401) region of RILP. ( d ) A diagram indicates the domain arrangement of RalGDS and the various truncation constructs. ( e ) MCF7 cells were transfected with myc-RalGDS, myc-RalGDS(GEF) or myc-RalGDS(RBD), respectively. The resulted cell lysates were subjected for GST-pulldown assays using GST-RILP. The results demonstrated that RILP interacts with the GEF domain of RalGDS. ( f ) MCF7 cells were co-transfected with HA-RILP and myc-RalGDS, myc-RalGDS(GEF) or myc-RalGDS(RBD), respectively. The resulted cell lysates were subjected for co-immunoprecipitation assays using rabbit anti-myc tag antibody, the immuno-complexes were revealed by western blot using anti-HA tag or anti-myc tag antibodies (9E10). The results confirmed that RILP interacts with the GEF domain of RalGDS
Pgbkt7 Vector Containing Rilp Fused To The Gal4 Dna Binding Domain, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+pact2/pgbkt7/pmc04632296-153-11-49
Average 90 stars, based on 1 article reviews
pgbkt7 vector containing rilp fused to the gal4 dna-binding domain - by Bioz Stars, 2026-10
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Image Search Results


RILP interacts with RalGDS. ( a ) AH109 yeast cells expressing pGBKT7-RILP, pGBKT7-RILP(1–198) or pGBKT7-RILP(199–401) was mated with Y187 yeast cells expressing pACT2-RalGDS(237–914), respectively. Growth on DDO (-Leu/-Trp) media indicated diploids expressing both plasmids. Growth on QDO (-Leu/-Trp/-His/-Ade) media indicated positive interaction between two hybrid proteins. ( b ) The endogenous RalGDS in MCF cell lysates binds to GST-RILP fusion protein as revealed by GST-pulldown using immobilized GST-RILP. ( c ) MCF7 cells were transfected with myc-RalGDS, and the resulted cell lysates were subjected for GST-pulldown assays using GST-RILP, GST-RILP(1–198) or GST-RILP(199–401), respectively. The results showed that RalGDS binds to full-length RILP and N-terminal (1–198) but not C-terminal (199–401) region of RILP. ( d ) A diagram indicates the domain arrangement of RalGDS and the various truncation constructs. ( e ) MCF7 cells were transfected with myc-RalGDS, myc-RalGDS(GEF) or myc-RalGDS(RBD), respectively. The resulted cell lysates were subjected for GST-pulldown assays using GST-RILP. The results demonstrated that RILP interacts with the GEF domain of RalGDS. ( f ) MCF7 cells were co-transfected with HA-RILP and myc-RalGDS, myc-RalGDS(GEF) or myc-RalGDS(RBD), respectively. The resulted cell lysates were subjected for co-immunoprecipitation assays using rabbit anti-myc tag antibody, the immuno-complexes were revealed by western blot using anti-HA tag or anti-myc tag antibodies (9E10). The results confirmed that RILP interacts with the GEF domain of RalGDS

Journal: Cell Death & Disease

Article Title: RILP suppresses invasion of breast cancer cells by modulating the activity of RalA through interaction with RalGDS

doi: 10.1038/cddis.2015.266

Figure Lengend Snippet: RILP interacts with RalGDS. ( a ) AH109 yeast cells expressing pGBKT7-RILP, pGBKT7-RILP(1–198) or pGBKT7-RILP(199–401) was mated with Y187 yeast cells expressing pACT2-RalGDS(237–914), respectively. Growth on DDO (-Leu/-Trp) media indicated diploids expressing both plasmids. Growth on QDO (-Leu/-Trp/-His/-Ade) media indicated positive interaction between two hybrid proteins. ( b ) The endogenous RalGDS in MCF cell lysates binds to GST-RILP fusion protein as revealed by GST-pulldown using immobilized GST-RILP. ( c ) MCF7 cells were transfected with myc-RalGDS, and the resulted cell lysates were subjected for GST-pulldown assays using GST-RILP, GST-RILP(1–198) or GST-RILP(199–401), respectively. The results showed that RalGDS binds to full-length RILP and N-terminal (1–198) but not C-terminal (199–401) region of RILP. ( d ) A diagram indicates the domain arrangement of RalGDS and the various truncation constructs. ( e ) MCF7 cells were transfected with myc-RalGDS, myc-RalGDS(GEF) or myc-RalGDS(RBD), respectively. The resulted cell lysates were subjected for GST-pulldown assays using GST-RILP. The results demonstrated that RILP interacts with the GEF domain of RalGDS. ( f ) MCF7 cells were co-transfected with HA-RILP and myc-RalGDS, myc-RalGDS(GEF) or myc-RalGDS(RBD), respectively. The resulted cell lysates were subjected for co-immunoprecipitation assays using rabbit anti-myc tag antibody, the immuno-complexes were revealed by western blot using anti-HA tag or anti-myc tag antibodies (9E10). The results confirmed that RILP interacts with the GEF domain of RalGDS

Article Snippet: In brief, the yeast strain AH109 harboring the pGBKT7 vector containing RILP fused to the Gal4 DNA-binding domain was mated with 6.05 × 10 7 independent yeast colonies (strain Y187) pretransformed with a human fetal brain cDNA library fused to the Gal4 DNA activation domain in the pACT2 vector (BD Biosciences).

Techniques: Expressing, Transfection, Construct, Immunoprecipitation, Western Blot